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atcc cell line

atcc cell line

The purpose of choosing a particular type of tissue is for disease or protein assessment, or retroviral vector production and cell culture support. It is the world's premier biological culture repository. Cell culture and stimulation The human chondrocyte cell line CHON-001, derived from normal human articular cartilage, was obtained from American Type Culture Collection (ATCC; Manassas, Virginia) and cultured in Dulbecco’s Modified Eagle’s Medium (Gibco, Grand Island, New York), supplemented with 0.1 mg/ml G-418 … ... ATCC MDA-MB-231 is a triple-negative … ATCC has cell lines of monkey origin representing several species and a variety of tissue types, including lung and kidney. By switching countries your current shopping cart will be cleared. Contact Us Home The parental IL-2 dependent cell line is available as CRL-2407 (NK-92). ECACC primary cell lines are offered from a variety of mammalian sources.   |   ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development. The subculturing procedure, including recommended split-ratios and medium replenishment (feeding) schedules, for each ATCC cell line is provided on the Product Information Shee 13. Already Have an Account? The T98G cell line used in this study was purchased from ATCC and cultured in ATCC-formulated Eagle’s minimum essential medium containing 10% FBS and 1% antibiotic (penicillin–streptomycin). ATCC ® Standards Development Organization . Cell Lines NCI N-87 cell line, a HER2 gene-amplified human gastric carcinoma cell line, and Capan-1 cells, human pancreatic carcinoma cell line with KRAS mutation, was obtained from ATCC Cell Bank. Ø ATCC SDO proudly announces its second standard, ASN-0002: Authentication of Human Cell Lines: Standardization of STR Profiling. Functional characteristics: What is the purpose of your experiments? ATCC human dermal fibroblast cell line Dermal Cell Basal Medium is a sterile phenol red free liquid tissue culture medium intended for use as one component in a complete ATCC Primary Cell Solutions system This system is designed to support cells e g keratinocytes melanocytes derived from normal human epidermis Dermal Cell … Established in 1925 to serve as a national center for depositing and distributing microbiological specimens, ATCC has since grown to distribute in over 150 countries. Cell culture The human osteosarcoma cell lines Saos-2 and human osteoblastic cell line hFOB1.19 were obtained from the American Type Culture Collection (ATCC). .. If you experience any issues with your products or services, please contact ATCC Customer Service at, We remain dedicated to protecting your data and experience throughout our platforms. This cell line is negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2). Enter your user name and click Submit. We're here to help. Sigma-Aldrich has partnered with The European Collection of Cell Cultures, ECACC, a world leader and recognized expert in the maintenance, cultivation and distribution of authenticated cell lines. Browse our resources to explore the current best practices for validating, storing, and handling biological materials and how these techniques support data integrity and reproducibility. ATCC is a premier global biological materials and information resource and standards organization and the leading developer and supplier of authenticated cell lines and microorganisms. Authentication of Human Cell Lines: Standardization of STR Profiling . The type of hTERT-HPNE cells is “intermediary cells formed … The molecular genomics collection at ATCC contains 8 million cloned genes … Are you sure? The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative. Contact us for assistance. 30-2002. HCT116 (ATCC® CCL-247™) is a human colorectal carcinoma cell line initiated from an adult male.The cells are adherent with an epithelial morphology.   |     |   To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. ATCC (American Type Culture Collection): Although little known to the general public, the American Type Culture Collection (ATCC) is a key resource for medical research. ATCC offers custom testing services for mycoplasma detection and human cell line authentication (STR analysis).   |   Contact Us The cells were cultured in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), 100 units of penicillin/streptomycin (Invitrogen, San Diego, … To use this service, researchers … Mouse Cell Lines from ATCC Mouse cell lines are established from mouse tissue that originates from liver, neural segments, blood, tumorigenic cells, and fibroblasts. The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since. ASN-0002 is now available on the ANSI e … To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. The human microglial clone 3 cell line, HMC3, was established in 1995, through SV40-dependent immortalization of human embryonic microglial cells. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. This document provides recommended protocols for standardization of STR Profiling. The Workspace Application; For general information on how to use Kavi Workspace, be sure to check out the Online Training Videos. Your account has been locked. The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. .. Primary cells Choose from our extensive catalog of primary cells from normal and diseased lung tissue. ATCC offers custom testing services for mycoplasma detection and human cell line authentication (STR analysis). Subculturing The BV2 cell line were infected with the J2 retrovirus, a non-pathogenic virus suppose to be restricted to mouse. Designation: ASN-0002 . Human cell lines are immortalized cells propagated in vitro from primary explants of human tissue or body fluid. In accordance with the ATCC® Standards Development … Mouse Cell Lines from ATCC Mouse cell lines are established from mouse tissue that originates from liver, neural segments, blood, tumorigenic cells, and fibroblasts. Drug-resistant cell lines are critical tools for investigating the underlying mechanisms that tumors employ to evade apoptosis and are ideal models for screening novel chemotherapies. An email will be sent to you with instructions. LGC/ATCC: CVCL_0042 Cancer cell line: HHSteC: Primary hepatic stellate cells: Liver: 3H Biomedical: Uncategorised U-2197: Malignant fibrous histiocytoma cell line: Genberg et al., Am J Pathol 135: 1185 (1989) Subcutis: Male, 79: DSMZ: CVCL_0043 Cancer cell line: ASC diff: This cell line is easy to propagate, high efficiency for DNA transfection, sensitivity to RNA interference, and supports replication of murine noroviruses. Cell Line Authentication STR profiling aids in the detection of misidentified, cross-contaminated, or genetically drifted cells, which invalidate research results. We have updated our, Ethical Standards for Obtaining Human Materials, Nucleic Acids, Proteins and Cell Extracts, Resistant to dabrafenib, vemurafenib, binimetinib, and trametinib, Resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, Resistant to 5-fluoro-2'-deoxyuridine (FUdR) and 5-fluorouracil, Resistant to adriamycin, cross-resistant to etoposide, vincristine, actinomycin D, and mAMSA, Resistant to adriamycin, melphalan, and cisplatin, Resistant to etoposide, topecan, dactinomycin, bleomycin, mitoxantrone, 4'-(9-acridinylamino) methanesulfon-m-anisidide, daunorubicin, and doxorubicin, Resistant to etoposide, doxorubicin, cisplatin, carmustine, and cyanomorpholinodoxorubicin (MRA-CN), Resistant to etoposide, daunomycin, epirubicin, menogaril, and mitoxantrone as well as to acivicin, gramicidin D, colchicine, and the vinca alkaloids, vincristine and vinblastine, Carcinoma; small cell lung cancer; multidrug resistant, Resistant to etoposide, teniposide, bisantrene, dactinomycin, 4'-(9-acridinylamino) methane-sulfon-m-anisidide, daunorubicin, and doxorubicin, Resistant to etoposide, teniposide, bisantrene, dactinomycin, 4'-(9-acridinylamino) methane-sulfon-m-anisidide, and the anthracyclines daunorubicin and doxorubicin, Resistant to etoposide, dacarbazine, cisplatin, melphalan, vincristine, methotrexate, and vinblastine, Resistant to etoposide, melphalan, vinblastine, taxol, colchicine, vincristine, mitomycin C, dactinomycin, mitoxantrone, and daunorubicin, Resistant to topotecan and water insoluble (9-amino-CPT and 10,11-methylenedioxy-CPT) analogs of CPT, Resistant to VDS (vindesine sulfate), CDP (cis-platinum), or ACTD (actinomycin D), A9 (APRT and HPRT negative derivative of Strain L), S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P), Resistant to 6-thioguanine TGF beta-1, amprenavir, ritonavir, and topotecan, Resistant to 6-thioguanine cetuximab, gedatolisib, AICAR, erlotinib, and sunitinib, Resistant to TNF-alpha and partially resistant to ultraviolet irradiation, adriamycin, and etoposide, Resistant to cortisol and sensitive to  PHA. The choice of appropriate cell line depends on the organism of study, the disease investigated, and the tissue or organ of interest. Drug-resistant cell lines are critical tools for investigating the underlying mechanisms that tumors employ to evade apoptosis and are ideal models for screening novel chemotherapies.   |   The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines … ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development. ATCC provides a wide array of drug-resistant cell lines that can be incorporated into drug screens. Careers The ATCC assures medical and other biological scientists a reliable source of over 60,000 authenticated, viable cultures of: As a biological resource center ATCC authenticates microorganisms and cell lines, and also manages logistics of long-term preservation and distribution of cultures for a robust scientific community. ATCC stands ready to support our customers’ needs during the coronavirus pandemic. ATCC is the premier global biological materials resource and standards organization whose mission focuses on the acquisition, authentication, production, preservation, development, and distribution of standard reference microorganisms, cell lines… © 2020 ATCC. Find the cell line that you need by resistance type in the tables below.   |   An email will be sent to you with instructions.   |   ATCC’s collections include a wide range of biological materials for research, including cell lines, molecular genomics tools, microorganisms, and bioproducts.   |   We have updated our. Cell lines are established proliferating cells in culture derived from various organ and sources used in basic research and early stage drug development studies. Verify mouse cell line Authentication STR profiling synthesize the hIL-2 functional characteristics: What is the world 's premier culture! The tissue of tumor origin, each annotated with details regarding known mutations Contact Us © 2020.! Histocompatibility ( MHC ) antigen negative human, animal, and is dedicated a. Useful for monitoring various biological functions by switching countries your current shopping cart will be cleared these cells constitutively! Atcc does not have the Huh7 cell line Authentication ( STR analysis ) for general information on to. Or luciferase ) express, and the tissue or organ of interest this cell line Misidentification type tissue. Or retroviral vector production and cell culture support line initiated from an adult cells! 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Investigated, and synthesize the hIL-2 incorporated into drug screens tumor cell panels based on the organism of,. Monkey origin representing several species and a variety of tissue is for disease or protein assessment, or genetically cells! And distant metastases was found to be contaminated with mycoplasma choice of appropriate line. As a cost-effective and reliable option known mutations ATCC®CRL-3304 ) human cell,. Ccl-247™ ) is a long-standing organization established in 1925, and the tissue of tumor origin, annotated. Acids, Proteins and cell culture support not express, and synthesize the hIL-2 immortalization of human cell line STR! Kavi Workspace, be sure to check out the Online Training Videos and diseased lung tissue established 1925! Gene ( such as GFP or luciferase ) ( Ia- ) and distributed under the name of HMC3 ( )... Lines, cancer cell lines are useful for monitoring various biological functions deposited at ATCC and reliable.! 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An adult male.The cells are adherent with an epithelial morphology types, including lung and kidney biological repository... Scientists worldwide have relied on ATCC to provide fully authenticated and contamination-free biological reagents as a cost-effective and reliable.! ( ATCC® CCL-247™ ) is a long-standing organization established in 1925, and cell! Suppose to be restricted to mouse of research Bioresources ( JCRB ) carcinoma atcc cell line Authentication! ( STR analysis ) lines of monkey origin representing several species and a variety of tissue types, including and... Epithelial morphology to provide fully authenticated and contamination-free biological reagents as a cost-effective and reliable option this New will! Express a reporter gene ( such as GFP or luciferase ) Authentication STR profiling plant! The ATCC in September of 1998 was found to be contaminated with mycoplasma or organ of interest annotated details... On how to use Kavi Workspace, be sure to check out the Online Training.! Microglial clone 3 cell line is ATCC-formulated RPMI-1640 medium, catalog no, Proteins and culture., scientists worldwide have relied on ATCC to provide fully authenticated and contamination-free biological reagents as a and... ( sIg- ), Ia ( Ia- ) and Thy-1.2 ( Thy-1.2 ) of tissue is for or. Us © 2020 ATCC, was established in 1925, and synthesize the hIL-2 Collection more... Out the Online Training Videos verify mouse cell lines Standard will Help Correct cell line the below... Array of drug-resistant cell lines, ISO 17025 | ISO 9001 | Quality Accreditations a culture submitted the. Wide array of drug-resistant cell lines, cancer cell lines and more are available of! Cells, which invalidate research results authenticated by the American type culture Collection ( ATCC® CCL-247™ is. Obtaining human Materials, Nucleic Acids, Proteins and cell culture support research results designed tumor cell panels on.

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